Transcriptional repression is an important mechanism by which genes can be regulated. This database stems from our investigations into Arabidopsis TOPLESS (TPL) and the four TPL-related proteins (TPR1-4), which belong to the Gro/Tup1 class of co-repressors first identified in Drosophila and yeast, respectively. TPL/TPR proteins interact with a broad range of transcription factor families, many of which are transcriptional repressors, with roles in diverse developmental processes including hormone signalling and stress responses.

We recently published the interactome for the TPL/TPR proteins (Causier et al. (2012) Plant Physiol. 158: 423). Here we present that interactome, together with previously published TPL interactions from various species. We also list reported interactions for other plant co-repressors including LUG/LUH, SEU/SLK, the LUG-SEU complex and SAP18 family proteins.

The TPL/TPR interactome (Causier et al., 2012) was established through two large-scale yeast two-hybrid approaches - whole plant library screens and screens of an arrayed library of Arabidopsis transcription factors:

Whole plant library screens

AGI	Identity		TPL	TPR1	TPR2	TPR3	TPR4	RDs
AT3G02550	LBD41							LDLTLRL
AT5G67420	LBD37							LDLSL
AT1G50640	ERF3	AP2/ERF						FDLNFPP	*
AT3G15210	ERF4	AP2/ERF						LELSL/LDLNLPP	*
AT3G20310	ERF7	AP2/ERF						FDLNFPP	*
AT1G53170	ERF8	AP2/ERF						LELSL/LDLDLNLAP	*
AT1G28370	ERF11	AP2/ERF						LDLDLNFPP	*
AT1G18330	EPR1	MYB						partial
AT2G16720	MYB7	MYB						LNLEL
AT4G34990	MYB32	MYB						LDLNLEL
AT5G67300	MYB44	MYB						LSLSL
AT3G50060	MYB77	MYB						LSLSL
AT3G52250	MYB/HD	MYB						partial
AT5G17300	MYB (EPR-like)	MYB						LKLELFP	*
AT1G46480	WOX4	HD						TLELFPL
AT1G27730	ZAT10	ZnF						FDLNIPP
AT5G04340	ZAT6	ZnF						LCLML/DLNIPP
AT1G08290	WIP3	ZnF						partial
AT2G42410	ZFP11	ZnF						LDLELRL
AT1G30810	Zn finger TF	ZnF						KLFGV/FDLNIDL
AT2G28200	ZAT5	ZnF						LPLDLNLP
AT2G27110	FRS3	ZnF						DLNLP
AT3G50870	MONOPOLE/GATA18	ZnF						partial
AT1G17380	JAZ5							DLNEPT/LDLRL
AT1G72450	JAZ6							DLNEPT/LELKL
AT3G23030	IAA2	AUX/IAA						LCLGL	*
AT3G23050	IAA7/AXR2	AUX/IAA						LCLGL/LMLNL	*
AT2G22670	IAA8	AUX/IAA						LRLGL
AT3G04730	IAA16	AUX/IAA						LRLGL/LKLNL
AT1G51950	IAA18	AUX/IAA						LELKL
AT4G29080	IAA27	AUX/IAA						LRLGL
AT1G04250	AXR3	AUX/IAA						LCLGL/LKLNL
AT5G62000	ARF2	ARF						LSLKL/RLFGI
AT4G23980	ARF9	ARF						RLFGI
AT3G61830	ARF18	ARF						RLFGF
AT1G25560	TEM1	AP2/ERF						RLFGV
AT2G28550	TOE1	AP2/ERF						LDLNL	*
AT5G60120	TOE2	AP2/ERF						LDLNL/LDLNL	*
AT2G36080	B3 TF							RLFGV
AT5G55040	BROMO TF							LELIL
AT1G58100	TCP8	TCP						partial	*
AT3G63180	TKL							partial
AT1G78700	BEH4							LELTL
AT4G36990	HSF4							KLFGV
AT2G47070	SPL1							LTLNL	*
AT1G80490	TPR1							partial
AT3G15880	TPR4							none
AT5G44800	PKR1							LPLFL
AT3G16840	HELICASE							partial
AT3G29575	AFP3							LDLGLSL
AT3G02140	AFP4							LTLGLSL
AT2G19520	FVE							partial
AT5G11530	EMF1							partial
AT3G24440	VRN5							partial
AT3G44630	TIR-NBS-LRR							partial
AT4G34370	ATARI1							LDLNLP
AT5G55170	SUMO3							partial
AT4G32030	unknown							LLLRL/DLNMAP
AT1G12120	unknown							DLNSTP
AT1G80610	unknown							DLNMP
AT5G57340	hypothetical							LELTL
AT4G32295	unknown							LSLRL
AT5G42530	unknown							LFLLLLL
AT5G14970	unknown							partial
AT3G17900	unknown							LELLPL

 

Arrayed TF library screens

AGI	Identity	TF	TPL	TPR1	TPR2	TPR3	TPR4	RDs
AT2G28550	TOE1	AP2/ERF						LDLNL	*
AT1G04100	IAA10	AUX/IAA						LDLALGL
AT3G23030	IAA2	AUX/IAA						LCLGL	*
AT1G77850	ARF17	ARF						partial
AT5G60120	TOE2	AP2/ERF						LDLNL/LDLNL	*
AT4G14560	IAA1	AUX/IAA						LRLGL
AT4G28640	IAA11	AUX/IAA						LELGLTLSL
AT2G37630	AS1	MYB						LELQL
AT5G24800	BZIP9	bZIP						partial
AT2G16770	BZIP23	bZIP						none
AT1G13260	RAV1	AP2/ERF						RLFGV
AT2G47070	SPL1	SBP						LTLNL	*
AT3G11580	AP2/B3	AP2/ERF						RLFGV
AT3G15540	IAA19	AUX/IAA						LRLGL
AT4G11660	HSFB2B	HSF						RLFGV
AT1G51120	AP2/B3	AP2/ERF						MLFGV
AT4G36920	AP2	AP2/ERF						DLNDAP/LDLSL
AT4G00540	MYB3R2	MYB						LDLTLGL
AT3G15030	TCP4	TCP						LRLSL
AT2G45660	SOC1	MADS						partial
AT4G37260	MYB73	MYB						LSLSL
AT2G33310	IAA13	AUX/IAA						LELGLGLSL
AT1G68670	MYB family	MYB						LPLCLEL
AT2G33550	GT-2 RELATED	MYB						LSLGL/LNLKL
AT5G13790	AGL15	MADS						LQLGL
AT2G37740	ZFP10	ZnF						LDLELRL
AT5G17300	RVE1	MYB						LKLELFP	*
AT1G66140	ZFP4	ZnF						LNLSL/LTLPL/LTLKL
AT1G04240	IAA3/SHY2	AUX/IAA						LRLGL
AT5G43700	IAA4	AUX/IAA						LRLGL
AT5G49300	GATA16	ZnF						none
AT1G50680	AP2/B3	AP2/ERF						MLFGV
AT3G20310	ERF7	AP2/ERF						FDLNFPP	*
AT1G50640	ERF3	AP2/ERF						FDLNFPP	*
AT4G28530	NAC074	NAC						partial
AT1G53230	TCP3	TCP						LRLSL
AT4G35610	Zn finger	ZnF						DLNVEP/FDLNKSP
AT4G17460	HAT1	HD						LGLSLSL/LQLNL
AT1G28360	ERF12	AP2/ERF						LSLDLNHLP/DLNEPP
AT2G38300	TF (G2-like)	MYB						LDLSLKL/LSLSL/LDLTL
AT3G15210	ERF4	AP2/ERF						LELSL/LDLNLPP	*
AT3G10490	NAC51/52	NAC						partial
AT3G10480	NAC50	NAC						partial
AT4G18390	TCP2	TCP						partial
AT5G59340	WOX2	HD						TLPLFP
AT2G44410	Zn binding	ZnF						LDLNQEP
AT2G31720	TF (B3)							LDLELRL
AT4G37790	HAT22	HD						LVLGLGL/LTLSL
AT3G16500	IAA26/PAP1	AUX/IAA						LELRL
AT5G25890	IAA28	AUX/IAA						LELRL
AT1G03800	ERF10	AP2/ERF						DLNASP
AT1G73100	SDG19							partial
AT2G41940	ZFP8	ZnF						LDLHL
AT1G28370	ERF11	AP2/ERF						LDLDLNFPP	*
AT1G53170	ERF8	AP2/ERF						LELSL/LDLDLNLAP	*
AT3G61970	NGA2	AP2/ERF						RLFGV/LQLRL
AT5G16560	KAN	MYB						partial
AT5G06250	AP2/B3	AP2/ERF						RLFGV
AT5G46590	NAC096	NAC						partial
AT1G18860	WRKY61	WRKY						partial
AT3G12730	MYB family	MYB						partial
AT4G03250	HD-ZIP							none
AT5G63470	NF-YC4							partial
AT1G08320	bZIP21	bZIP						partial
AT5G51990	CBF4	AP2/ERF						partial
AT4G32890	GATA9	ZnF						partial
AT4G30935	WRKY32	WRKY						partial
AT5G06960	OBF5	bZIP						partial
AT1G62300	WRKY6	WRKY						partial
AT3G44460	DPBF2/bZIP67	bZIP						LSLTL/LELEL
AT1G25550	MYB family	MYB						LPLCLEL
AT3G15500	NAC3/NAC55	NAC						partial
AT3G45150	TCP16	TCP						partial
AT1G35560	TCP family	TCP						partial
AT4G26930	MYB97	MYB						partial
AT3G12250	TGA6/bZIP45	bZIP						partial
AT1G71030	MYBL2	MYB						LILKL/TLLLFR
AT1G66230	MYB20	MYB						LDLLL
AT3G23250	MYB15	MYB						partial
AT5G24110	WRKY30	WRKY						none
AT3G23050	IAA7/AXR2	AUX/IAA						LCLGL/LMLNL	*
AT3G16350	MYB family	MYB						KLFGV/LELSL/LSLRL
AT1G58100	TCP8	TCP						partial	*
AT5G62020	HSFB2A	HSF						RLFGV
AT3G47620	TCP14	TCP						partial
AT3G21890	Zn finger	ZnF						none
AT4G18770	MYB98	MYB						none
AT5G17430	BABYBOOM	AP2/ERF						partial

In each of the tables above, the first column provides the AGI number for each factor. The second column indicates the identity of the factor, if known. Factors highlighted in grey in this column represent those that are known to act as transcriptional repressors. The third column indicates the transcription factor family to which each factor belongs. Interactions between these factors and the TPL/TPR proteins are represented by dark grey boxes in the appropriate columns. The RD column shows the sequence of any known functional repression domain found in the TPL/TPR-interacting factors. An asterix to the right-hand side of each table indicate those factors isolated in both the whole plant library and arrayed transcription factor screens.

 

Other published co-repressor interactions

TPL/TPR family

*At – Arabidopsis thaliana; Am – Antirrhinum majus; Pp – Physcomitrella patens; Zm – Zea mays

Experiments: Y2H – yeast two-hybrid; PD – pull-down; Co-IP – co-immunoprecipitation; TAP – tandem affinity purification; BiFC – biomolecular fluorescence complementation

Refs:

• A - Arabidopsis Interactome Mapping Consortium (2011) Science 333: 601.
• C – Causier et al. (2012) Plant Signalling and Behavior 7: in press
• G – Gallavotti et al. (2010) Development 137: 2849.
• K – Kieffer et al. (2006) Plant Cell 18: 560.
• P – Pauwels et al. (2010) Nature 464: 788.
• S – Szemenyei et al. (2008) Science 319: 1384.
• Z – Zhu et al. (2010) PNAS 107: 13960.

 

LUG/LUH family

*At – Arabidopsis; Am - Antirrhinum

Experiments: Y2H – yeast two-hybrid; PD – pull-down; BRET – bioluminescence resonance energy transfer Refs:

• G – Gregis et al. (2006) Plant Cell 18: 1373.
• N – Navarro et al. (2004) Development 131: 3649.
• St – Stahle et al. (2009) Plant Cell 21: 3105.
• S1 – Sridhar et al. (2004) PNAS 101: 11494.

 

SEU/SLK family

*At – Arabidopsis; Am – Antirrhinum

Experiment: Y2H – yeast two-hybrid; Y3H – yeast three-hybrid; PD – pull-down; BRET – bioluminescence resonance energy transfer

Refs:

• G – Gregis et al. (2006) Plant Cell 18: 1373.
• N – Navarro et al. (2004) Development 131: 3649.
• St – Stahle et al. (2009) Plant Cell 21: 3105.
• S1 – Sridhar et al. (2004) PNAS 101: 11494.
• S2 – Sridhar et al. (2006) Development 133: 3159.

 

LUG/SEU complexes

*At – Arabidopsis; Am – Antirrhinum

Experiment: Y4H – yeast four-hybrid; Y3H – yeast three-hybrid; PD – pull-down

Refs:

• G – Gregis et al. (2006) Plant Cell 18: 1373.
• N – Navarro et al. (2004) Development 131: 3649.
• S2 – Sridhar et al. (2006) Development 133: 3159.

 

Arabidopsis SAP18

Experiment: Y2H – yeast two-hybrid; PD – pull-down; EMSA – electrophoretic mobility shift assay; Co-IP – co-immunoprecipitation; BiFC – biomolecular fluorescence complementation

Refs:

• H – Hill et al. (2008) Plant J 53: 172.
• So – Song & Galbraith (2006) Plant Mol Biol 60: 241.
• L – Liu et al. (2009) Dev Cell 16: 711.